The prior measurement of cortisol had a different methodology and was done using Luminescent Immunoassay (LIA). Therefore, many changes can be attributed to methodology, the platform used and differing reagents. The new reference ranges are provided in the Salimetrics kit and apply to this new methodology. To further validate the new reference ranges, we tested a population of apparently healthy patients aged 18-65, whose prior cortisol and DHEA samples reflected normal ranges and followed the normal diurnal pattern. Even with the expansion of the age group, extrapolation of the results showed no statistical change. The Salimetics kit and these reference ranges are considered FDA equivalent.